Detection of protein binding abnormalities in euthyroid hyperthyroxinemia.

Differentiating various abnormalities of thyroxin binding to serum proteins by radioelectrophoresis of thyroxin and immunoassay of binding proteins.

Using the simple method of protein analysis described here, we could identify thyroxin (T4)-binding-protein abnormalities in euthyroid patients with hyperthyroxinemia or hypothyroxinemia. Serum incubated with [125I]thyroxin was analyzed by agarose gel electrophoresis, with bromphenol blue staining of protein. The relative distribution of radioactive T4 was determined for each binding protein--t...

Instability of calibration curves of liquid-chromatographic techniques with electrochemical detection: role of the detector.

CLINICALCHEMISTRY, Vol. 34, No. 3, 1988 599 2. Stockigt JR, De Garis M, Barlow JW. “Unbound analog” methods for free T4: a note of caution. N Engi J Med 1982;307:126. 3. De Nayer P, Malvaux P, Beckers C. Familial dysalbuminemic hyperthyroxinemia (FDH): inadequacy of the “analog” methods for assaying free T4 levels. Eur J Nuci Med 1984;9:284-5. 4. Stockigt JR, Dyer SA, Mohr VS. White EL, BarlowJ...

Dysprealbuminemic hyperthyroxinemia in a patient with hyperthyroid graves disease.

Rare mutant forms of circulating albumin and prealbumin [transthyretin (TTR)] have increased binding affinity for thyroxine (T4). Patients with these variant plasma proteins, as a result of inherited mutations or as a paraneoplastic phenomenon, typically present with increased serum total T4 and, by some assay methodologies, an increased free T4 as well. Although these individuals are, in fact,...

Clinical, Genetic, and Protein Structural Aspects of Familial Dysalbuminemic Hyperthyroxinemia and Hypertriiodothyroninemia

Familial dysalbuminemic hyperthyroxinemia (FDH-T4) and hypertriiodothyroninemia (FDH-T3) are dominantly inherited syndromes characterized by a high concentration of thyroid hormone in the blood stream. The syndromes do not cause disease, because the concentration of free hormone is normal, but affected individuals are at risk of erroneous treatment. FDH-T4 is the most common cause of euthyroid ...

Euthyroid hyperthyroxinemia: a case report.